restriction endonuclease
限制性内切酶：一种酶
常用释义
英式发音
美式发音
基本释义
- 限制性内切酶：一种酶，能够在DNA分子内特定的位置上将其切割成片段。
例句
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1·Methods:DNA sequencing and restriction endonuclease reaction was used.方法：采用DNA测序及限制性酶切反应方法。
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2·After a restriction endonuclease cleavage, two expected smaller fragments were observed.经限制性内切酶反应，获得两个预期大小的片段。
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3·The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
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4·Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.然后对扩增产物进行限制性酶切反应；
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5·In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。
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6·Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
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7·Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
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8·Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
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9·Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;并对发现突变的基因进行分析。方法 应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
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10·Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。