isoelectric focusing
从而实现分离和纯化。
常用释义
英式发音
美式发音
基本释义
- 等电聚焦：一种生物化学技术，通过在电场中使蛋白质或其他生物分子在其等电点处聚焦，从而实现分离和纯化。
例句
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1·Isoelectric focusing may be used as another indication.等电聚焦可以用为另一指标。
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2·Haemoglobin polymorphism of Chinese miniature horses was examined by agarose gel isoelectric focusing.采用等电聚焦电泳对中国矮马进行了血红蛋白多态性的检测。
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3·The results showed that low ion intensity sample washing buffer improved the performance of isoelectric focusing.结果表明：采用低离子强度的缓冲液冲洗收集样品可以提高等电聚焦效果。
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4·After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
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5·Isoelectric focusing of STFC in thin-layer polyacrylamide gel obtained only one band as shown by Coomassie Blue staining.在等电点聚焦分析中该成分为一条考马斯亮蓝染色带。
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6·Capillary isoelectric focusing (CIEF) is a more rapid technique for separating proteins that the traditional gel-based technique.和传统的凝胶技术相比，毛细管等电聚焦(CIEF)是一种蛋白质的快速分离技术。
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7·Its molecular weight is 64,000 and isoelectric point around pH 4.2 as determined by SDS-PAGE and isoelectric focusing analysis respectively.SDS聚丙烯酰胺凝胶电泳测定分子量为64,000。等电聚焦电泳测得等电点在4.2左右。
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8·Methods: Saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.方法：采集33例正常人，26例牙周炎患者全唾液，运用聚丙烯酰胺凝胶等电聚焦方法观察其蛋白质等电点分布。
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9·Inheritance of PGI isozymes in chestnut species was analyzed using isoelectric focusing on thin layer polyacrylamide slab gels and single tree progeny method.采用超薄聚丙烯酰胺平板凝胶等电聚焦电泳和单株后代法，分析了栗属种的PGI同工酶的遗传。
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10·The keratin compositions of the human and animal hairs belonging to 6 orders, 15 families and 20 species was studied by polyacrylamide gel isoelectric focusing (PAGIEF).本文用聚丙烯酰胺等电聚焦（PAGIEF）对来自6个目、15个科、20种动物毛发角蛋白组分进行了研究。